Codon optimization, expression, purification, and functional characterization of recombinant human IL-25 in Pichia pastoris
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چکیده
منابع مشابه
Expression, Purification and Characterization of Human Recombinant Galectin 3 in Pichia pastoris
Background: Over the past century, the areas of genomics, proteomics and lipids have captured the attention of investigators worldwide. Carbohydrates, have recently received increased attention through the expanding field of glycobiology; probably because they are very complex and not encoded in the genome. Objectives: The purpose of this study was to express and purify recombinant human galec...
متن کاملexpression, purification and characterization of human recombinant galectin 3 in pichia pastoris
background: over the past century, the areas of genomics, proteomics and lipids have captured the attention of investigators worldwide. carbohydrates, have recently received increased attention through the expanding field of glycobiology; probably because they are very complex and not encoded in the genome. objectives: the purpose of this study was to express and purify recombinant human galect...
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Pancreatic duodenal hemeobox-1 (PDX1) is essential for the development of the embryonic pancreas and plays a key role in pancreatic beta-cell differentiation, maturation, regeneration, and maintenance of normal pancreatic beta-cell insulin-producing function. Purified recombinant PDX1 (rPDX1) may be a useful tool for many research and clinical applications, however, using the Escherichia coli e...
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* Correspondence: [email protected]
متن کاملexpression and purification of functionally active recombinant human alpha 1-antitrypsin in methylotrophic yeast pichia pastoris
human alpha 1-antitrypsin (aat) cdna was obtained from hepg2 cell lines. after pcr and construction of expression vector ppiczα-aat, human aat was expressed in the yeast pichia pastoris (p.pastoris) in a secretary manner and under the control of inducible alcohol oxidase 1 (aox1) promoter. the amount of aat protein in medium was measured as 60 mg/l 72 hr after induction with methanol. results i...
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ژورنال
عنوان ژورنال: Applied Microbiology and Biotechnology
سال: 2013
ISSN: 0175-7598,1432-0614
DOI: 10.1007/s00253-013-5264-4